Scientist, Protein Purification- Structural Biology
Employment Type: Full-Time
Reports To:Senior Principal Scientist, Structural BiologyPosition SummaryLoxo Oncology at Lilly conducts discovery research, clinical development and regulatory affairs for oncology, with the goal of creating a pipeline of therapies that have the potential to make a meaningful difference for patients. Loxo Oncology at Lilly was created in December 2019, combining the Lilly Research Laboratories oncology organization and Loxo Oncology, which was acquired by Lilly in early 2019. The unit has team members in Boulder, CO, Indianapolis, IN, New York City, South San Francisco, Stamford, CT, and Madrid, Spain.This position offers the opportunity to be part of Loxo's discovery teams focused on creating novel, highly specific and selective drugs for genetically identified targets. The Scientist, Protein Purification will employ modern cloning methods to support the gene-to-structure pipeline across multiple projects and project teams. This is an extremely important role where the right person will be able to have a real impact on cutting-edge science, making a real difference in patient's lives.Roles and Responsibilities of the Position** Provide recombinant protein expression support within a structural biology & enzymology setting.* Execute projects in an efficient manner, working within aggressive timelines.* Rapidly communicate scientific results and insights to the team.* Prepare reports for internal and external purposes including regulatory submissions.* Participate and lead project-based and technical team meetings to identify and solve problems, guide work towards project goals, communicate on-going work and progress, and plan forward thinking strategies.* Continually advance your understanding of current methodology and new/emerging technologies by reading scientific literature.* Proactively investigate new or different technologies, methods, and service providers to ensure our work is being done in a scientifically sound, efficient, and reasonable way.Required Qualifications and Background* BA/BS/MS/PhD in biology, genetics, biochemistry, molecular biology or a related field.* Ideal candidate will have with 5+ years hands-on experience, working in a gene to structure laboratory in a biotech/pharma setting, but less experienced, motivated individuals are encouraged to apply.* Expertise with FPLC protein purification techniques such as IMAC, SEC, IEX, HIC is required, ideally utilizing the Akta FPLC systems.* Expertise with protein characterization techniques such as Mass Spectrometry (Orbitrap, QTOF, hybrid traps) and Thermal Denaturation assays such as differential scanning fluorimetry (DSF) is highly desirable.* Familiarity with biophysical techniques such as SPR, ITC, Fluorescent binding assays, enzyme kinetic assays is desirable.* Experience with a variety of protein expression systems (E.coli, BV, mammalian, yeast) as well as modern cloning technologies e.g. Gibson assembly, gBlock utilization is a plus.* The candidate must be self-motivated but always maintain focus on why we are here and who is important i.e. the patients and their families/caregivers.* Excellent interpersonal skills with ability to communicate and interact well with colleagues, vendors, contractors, scientific advisors, and staff at all levels.* Desire to cross-train and flex between sub-groups (protein expression/purification/characterization) is a must.* Highly organized with excellent analytical, documentation and multi-tasking skills.* Experience across the gene-to-structure pipeline is a plus.Physical Demands / TravelThe physical demands of this job are consistent with light office duties.The physical demands here are representative of those that must be met by an employee to successfully perform the essential functions of this job.Work EnvironmentThis position's work environment is in an office and the lab.The work environment characteristics described here are representative of those encountered by an employee while performing the essential functions of this job.* To perform this job successfully, an individual must be able to perform each role and responsibility satisfactorily. The requirements listed above are representative of the knowledge, skill, and/or ability required. Reasonable accommodations may be made to enable individuals with disabilities to perform the essential functions.Loxo Oncology, Inc. is an EEO/Affirmative Action Employer and does not discriminate on the basis of age, race, color, religion, gender, sexual orientation, gender identity, gender expression, national origin, protected veteran status, disability or any other legally protected status.